Basic Science

How Lipogems® May Help Calm Joint Inflammation

Secretome from Human Micro-Fragmented Adipose Tissue Affects In Vitro Monocytes/Macrophages Inflammatory Activity by ICAM-1 Expression

Valentina Coccè, Eleonora Martegani, Francesca Paino, Luisa Doneda, Giulio Alessandri, Barbara Manfredi, Aldo Giannì, Emilio Ciusani, Elena Colombani, Carlo Tremolada, Augusto Pessina, Mediators of Inflammation, 2024

Important Notice: This Research Does Not Apply to Lipogems® Treatment

Technical considerations for isolated limb perfusion: A consensus paper

Andrew J. Hayes, David J. Coker, Lukas Been, Veerle WSM. Boecstaens, Sylvie Bonvalot, Franco De Cian, Luis de la Cruz-Merino, Carlos Duarte, Alexander Eggermont, Victor Farricha, Marco Fiore, Dirk Grünhagen, Robert Grützmann, Charles Honoré, Jens Jakob, Marko Hocevar, Winan van Houdt, Joseph Klauzner, Christoph Kettelhack, Angela Märten, Hector Martinez-Said, Maurice Matter, Audrey Michot, Maya Niethard, Elisabetta Pennacchioli, Lars E. Podleska, Gregorio Rabago, Marco Rastrelli, Sophie Reijers, Matilde Ribeiro, Matthias Schwarzbach, Hayden A. Snow, Miroslav Spacek, Stephan Stoldt, Alessandro Testori, Odysseas Zoras, Roger Olofsson Bagge, European Journal of Surgical Oncology, 2024

Understanding Micro-Fragmented Fat Tissue for Healing and Future Medical Applications

Micro fractured Adipose Tissue Graft (Lipogems), Regenerative Surgery and Potential Outcomes for Infectious and Cancer Diseases

Carlo Tremolada, Pierre Rocheteau, Carmelo Bisognano, Ofer Zeira, Giulio Alessandri, Journal of Stem Cell Research, 2023

Frozen Fat Tissue Yields Healthy Stem Cells for Future Use

Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis - a comparative study of isolation by tissue explant culture and enzymatic digestion

Jasmin Bagge, Per Hölmich, Freja Aabæk Hammer, Jan O. Nehlin, Kilian Vomstein, Lars Blønd, Lisbet Rosenkrantz Hölmich, Kristoffer Weisskirchner Barfod, Journal of Experimental Orthopaedics, 2023

Understanding Bone Healing Research Using Your Body's Own Fat Tissue

Effects of Autologous Microfragmented Adipose Tissue on Bone Healing: A Blinded, Prospective, Randomised Canine Clinical Trial

Luca Pennasilico, Caterina Di Bella, Sara Sassaroli, Alberto Salvaggio, Francesco Roggiolani, Angela Palumbo Piccionello, MDPI, 2023

Micro-Fragmented Adipose Tissue: A Potential New Approach for Brain Cancer Treatment

Micro-Fragmented Adipose Tissue as a Natural Scaffold for Targeted Drug Delivery in Brain Cancer

Alex Salagean, Adela Nechifor-Boila, Nosherwan Bajwa, Ylenia Pastorello, Mark Slevin, International Journal of Molecular Sciences, 2023

Understanding Lipogems®: What Research Shows About Fat Tissue for Healing

Microfractured Adipose Tissue Graft (Lipogems) and Regenerative Surgery

Carlo Tremolada, Journal of Orthopedic Research and Therapy, 2022

3D-Printed Fat Tissue Survives 150 Days in Animal Study

Long-term in vivo survival of 3D-bioprinted human lipoaspirate-derived adipose tissue: proteomic signature and cellular content

Karin Säljö, Peter Apelgren, Linnea Stridh Orrhult, Susann Li, Matteo Amoroso, Paul Gatenholm, Lars Kölby, Adipocyte, 2022

Lipoaspirate Shows In Vitro Potential for Wound Healing

Chiara Ceresa, Alessia Borrone, Letizia Fracchia, Maurizio Rinaldi, Alice Marchetti, Carlo Tremolada, Michela Bosetti, Pharmaceutics, 2022

Understanding How Fat-Derived Cells Support Blood Vessel Formation

CD146+ Pericytes Subset Isolated from Human Micro-Fragmented Fat Tissue Display a Strong Interaction with Endothelial Cells: A Potential Cell Target for Therapeutic Angiogenesis

Ekta Manocha, Alessandra Consonni, Fulvio Baggi, Emilio Ciusani, Valentina Cocce, Francesca Paino, Carlo Tremolada, Arnaldo Caruso, Giulio Alessandri, International Journal of Molecular Sciences, 2022

Understanding New Research on Fat Tissue for Wound Healing

Lipoaspirate Shows In Vitro Potential for Wound Healing

Chiara Ceresa, Alessia Borrone, Letizia Fracchia, Maurizio Rinaldi, Alice Marchetti, Carlo Tremolada, Michela Bosetti, Pharmaceutics, 2022

Understanding How Processed Fat Tissue Supports Joint Healing

Characterization of Microfragmented Adipose Tissue Architecture, Mesenchymal Stromal Cell Content and Release of Paracrine Mediators

Enrico Ragni, Marco Viganò, Enrica Torretta, Carlotta Perucca Orfei, Alessandra Colombini, Carlo Tremolada, Cecilia Gelfi, Laura de Girolamo, Journal of Clinical Medicine, 2022

Fat Tissue Delivers Chemotherapy Directly to Cancer Sites in Dog Study

Case Report: Microfragmented Adipose Tissue Drug Delivery in Canine Mesothelioma: A Case Report on Safety, Feasibility, and Clinical Findings

Offer Zeira, Erica Ghezzi, Letizia Pettinari, Valentina Re, Davide M. Lupi, Silvia L. Benali, Simone Borgonovo, Giulio Alessandri, Francesco Petrella, Rita Paroni, Michele Dei Cas, Carlo Tremolada, Valentina Coccè, Augusto Pessina, Frontiers in Veterinary Science, 2021

Understanding Fat-Based Regenerative Treatments for Joint and Muscle Conditions

Adipose-Derived Stem/Stromal Cells, Stromal Vascular Fraction, and Microfragmented Adipose Tissue

Enrico Ragni, Marco Viganò, Paola De Luca, Edoardo Pedrini, Laura de Girolamo, 2021

Lab Study Compares Fat Tissue Preparations for Knee Cartilage Health

Comparison of Clinically Relevant Adipose Preparations on Articular Chondrocyte Phenotype in a Novel In Vitro Co-Culture Model

Lauren Kokai, Joseph Chen, Dong Wang, Sheri Wang, Francesco M. Egro, Benjamin Schilling, Hengyun Sun, Asim Ejaz, J. Peter Rubin, Jeffrey A. Gusenoff, Nam Vo, Kentaro Onishi, Gwendolyn Sowa, Stem Cells and Development, 2021

Understanding Fat-Based Regenerative Treatments for Joint Conditions

Adipose-Derived Stem/Stromal Cells, Stromal Vascular Fraction, and Microfragmented Adipose Tissue

Enrico Ragni, Marco Viganò, Paola De Luca, Edoardo Pedrini, Laura de Girolamo, 2021

Micro-Fragmented Fat Shows Best Cartilage Protection in Knee Arthritis Study

Micro-fragmentation is a valid alternative to cell expansion and enzymatic digestion of adipose tissue for the treatment of knee osteoarthritis: a comparative preclinical study

Giuseppe Filardo, Matilde Tschon, Francesco Perdisa, Silvia Brogini, Carola Cavallo, Giovanna Desando, Gianluca Giavaresi, Brunella Grigolo, Lucia Martini, Nicolò Nicoli Aldini, Alice Roffi, Milena Fini, Elizaveta Kon, Knee Surgery, Sports Traumatology, Arthroscopy, 2021

Understanding How Lipogems® Processing Preserves Your Body's Healing Cells

Microfragmented adipose tissue is associated with improved ex vivo performance linked to HOXB7 and b-FGF expression

Giulia Casari, Elisa Resca, Andrea Giorgini, Olivia Candini, Tiziana Petrachi, Maria Serena Piccinno, Elisabetta Manuela Foppiani, Lucrezia Pacchioni, Marta Starnoni, Massimo Pinelli, Giorgio De Santis, Filippo Selleri, Fabio Catani, Massimo Dominici, Elena Veronesi, 2021

Lab Study Shows Processed Fat Tissue Calms Inflammation in Rotator Cuff Cells

Autologous microfragmented adipose tissue reduces inflammatory and catabolic markers in supraspinatus tendon cells derived from patients affected by rotator cuff tears

Marco Viganò, Gaia Lugano, Carlotta Perucca Orfei, Alessandra Menon, Enrico Ragni, Alessandra Colombini, Paola De Luca, Pietro Randelli, Laura de Girolamo, International Orthopaedics, 2020

Understanding the Lipogems® Patent: Heart Sound Technology for Stem Cell Preparation

Method and Device for Preparing Non-Embryonic Stem Cells

Carlo Tremolada, Carlo Ventura, Milford Graves, United States Patent, 2020

Micro-Fragmented Fat Tissue Shows Promise for Cartilage Repair

Autologous Micro-Fragmented Adipose Tissue as Stem Cell-Based Natural Scaffold for Cartilage Defect Repair

Tengjing Xu, Xinning Yu, Quanming Yang, Xiaonan Liu, Jinghua Fang, Xuesong Dai, Cell Transplantation, 2019

What's Inside Your Own Fat Tissue That Helps Heal Knee Arthritis?

Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

Denis Polancec, Lucija Zenic, Damir Hudetz, Igor Boric, Zeljko Jelec, Eduard Rod, Trpimir Vrdoljak, Andrea Skelin, Mihovil Plecko, Mirjana Turkalj, Boro Nogalo, Dragan Primorac, Genes, 2019

Understanding How Micro-Fragmented Fat Tissue Fights Inflammation Long-Term

Long-Lasting Anti-Inflammatory Activity of Human Microfragmented Adipose Tissue

Sara Nava, Valeria Sordi, Luisa Pascucci, Carlo Tremolada, Emilio Ciusani, Offer Zeira, Moris Cadei, Gianni Soldati, Augusto Pessina, Eugenio Parati, Mark Slevin, Giulio Alessandri, Stem Cells International, 2019

Understanding the Lipogems® Device: How Your Fat Tissue Gets Prepared for Treatment

Device and Method for Preparing Adipose Tissue for Transplantation

Carlo Ferdinando Maria Tremolada, Antonio Bosetto, Paolo Pirazzoli, United States Patent, 2019

Fat Tissue Shows Promise as Drug Carrier in Lab Cancer Studies

Microfragmented human fat tissue is a natural scaffold for drug delivery: Potential application in cancer chemotherapy

Giulio Alessandri, Valentina Coccè, Fabio Pastorino, Rita Paroni, Michele Dei Cas, Francesco Restelli, Bianca Pollo, Laura Gatti, Carlo Tremolada, Angiola Berenzi, Eugenio Parati, Anna Teresa Brini, Gianpietro Bondiolotti, Mirco Ponzoni, Augusto Pessina, Journal of Controlled Release, 2019

Understanding the Lipogems® Patent: A Gentler Way to Process Your Body's Own Fat for Healing

Method for Preparing Tissue, Particularly Adipose Tissue, for Transplantation from Lobular Fat Extracted by Liposuction

Carlo Tremolada, United States Patent Application Publication, 2019

Understanding Your Body's Natural Healing Cells: A Review of Lipogems® Science

Mesenchymal Stromal Cells and Micro Fragmented Adipose Tissue: New Horizons of Effectiveness of Lipogems

Carlo Tremolada, Massimo Allegri, Mark Slevin, Journal of Stem Cells Research, Development and Therapy, 2019

Understanding How Fat Processing Methods Affect Healing Potential

Higher Pericyte Content and Secretory Activity of Microfragmented Human Adipose Tissue Compared to Enzymatically Derived Stromal Vascular Fraction

Bianca Vezzani, Isaac Shaw, Hanna Lesme, Li Yong, Nusrat Khan, Carlo Tremolada, Bruno Péault, Stem Cells Translational Medicine, 2018

How Micro-Fragmented Fat Tissue May Calm Joint Inflammation

Effect of microfragmented adipose tissue on osteoarthritic synovial macrophage factors

Francesca Paolella, Cristina Manferdini, Elena Gabusi, Laura Gambari, Giuseppe Filardo, Elizaveta Kon, Erminia Mariani, Gina Lisignoli, Journal of Cellular Physiology, 2018

Understanding How Lipogems® Processing Changes Fat Tissue Chemistry

Metabolomic changes in human adipose tissue derived products following non-enzymatic microfacturing

M. Garcia-Contreras, F. Messaggio, A. J. Mendez, C. Ricordi, 2018

Fat Tissue Injections Safely Reduce Arthritis Pain in 130 Dogs

Intra-Articular Administration of Autologous Micro-Fragmented Adipose Tissue in Dogs with Spontaneous Osteoarthritis: Safety, Feasibility, and Clinical Outcomes

Offer Zeira, Simone Scaccia, Letizia Pettinari, Erica Ghezzi, Nimrod Asiag, Laura Martinelli, Daniele Zahirpour, Maria P. Dumas, Martin Konar, Davide M. Lupi, Laurence Fiette, Luisa Pascucci, Leonardo Leonardi, Alistair Cliff, Giulio Alessandri, Augusto Pessina, Daniele Spaziante, Marina Aralla, Stem Cells Translational Medicine, 2018

Fat Processing System Isolates Healing Cells for Cancer Drug Delivery

A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications

Valentina Coccè, Anna Brini, Aldo Bruno Giannì, Valeria Sordi, Angiola Berenzi, Giulio Alessandri, Carlo Tremolada, Silvia Versari, Antonio Bosetto, Augusto Pessina, Unknown, 2018

Micro-Fragmented Fat Shows Promise in Reducing Sepsis Inflammation

Micro-fragmented fat injection reduces sepsis-induced acute inflammatory response in a mouse model

A. Bouglé, P. Rocheteau, M. Hivelin, A. Haroche, D. Briand, C. Tremolada, J. Mantz, F. Chrétien, British Journal of Anaesthesia, 2018

Understanding Lipogems® Technology: A Natural Approach to Healing

Mesenchymal Stem Cells and Regenerative Medicine: How Lipogems Technology Make Them Easy, Safe and More Effective to Use

Carlo Tremolada, 2017

Understanding Fat Tissue as a Source of Healing Cells

Adipose Tissue and Mesenchymal Stem Cells: State of the Art and Lipogems® Technology Development

Carlo Tremolada, Valeria Colombo, Carlo Ventura, Current Stem Cell Reports, 2016

Understanding How Lipogems® May Support Rotator Cuff Healing

Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability

Pietro Randelli, Alessandra Menon, Vincenza Ragone, Pasquale Creo, Sonia Bergante, Filippo Randelli, Laura De Girolamo, Umberto Alfieri Montrasio, Giuseppe Banfi, Paolo Cabitza, Guido Tettamanti, Luigi Anastasia, BioMed Research International, 2016

Understanding How Lipogems® May Help With Tissue Repair and Inflammation

Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells

Valentina Ceserani, Anna Ferri, Angiola Berenzi, Anna Benetti, Emilio Ciusani, Luisa Pascucci, Cinzia Bazzucchi, Valentina Coccè, Arianna Bonomi, Augusto Pessina, Erica Ghezzi, Offer Zeira, Piero Ceccarelli, Silvia Versari, Carlo Tremolada, Giulio Alessandri, 2016

Your Own Fat Tissue May Help Repair Damaged Cartilage

Human Lipoaspirate as Autologous Injectable Active Scaffold for One-Step Repair of Cartilage Defects

Michela Bosetti, Alessia Borrone, Antonia Follenzi, Fanuel Messaggio, Carlo Tremolada, Mario Cannas, Cell Transplantation, 2016

Fat Cell Secretions Calm Inflamed Brain Cells in Lab Study

The Adipose Mesenchymal Stem Cell Secretome Inhibits Inflammatory Responses of Microglia: Evidence for an Involvement of Sphingosine-1-Phosphate Signalling

Giovanni Marfia, Stefania Elena Navone, Loubna Abdel Hadi, Moira Paroni, Valeria Berno, Matteo Beretta, Roberta Gualtierotti, Francesca Ingegnoli, Vincenzo Levi, Monica Miozzo, Jens Geginat, Lorenzo Fassina, Paolo Rampini, Carlo Tremolada, Laura Riboni, Rolando Campanella, 2016

Understanding Lipogems®: How Clinical Success Led Scientists to Discover Why Fat Tissue Heals

Mesenchymal Stem Cells in Lipogems, a Reverse Story: from Clinical Practice to Basic Science

Carlo Tremolada, Camillo Ricordi, Arnold I. Caplan, Carlo Ventura, 2016

Understanding the Regenerative Power of Processed Fat Tissue

Characteristics and Properties of Mesenchymal Stem Cells Derived From Microfragmented Adipose Tissue

Stephana Carelli, Fanuel Messaggio, Alessandra Canazza, Danuta Maria Hebda, Filippo Caremoli, Elisa Latorre, Maria Grazia Grimoldi, Mattia Colli, Gaetano Bulfamante, Carlo Tremolada, Anna Maria Di Giulio, Alfredo Gorio, Cell Transplantation, 2015

Boosting Stem Cell Potential Through Chemical Conditioning

Chemical Preconditioning Process for Cell Material to Obtain Chemical Epigenetic Reprogramming and Pluripotency Expression

Carlo Tremolada, Carlo Ventura, United States Patent Application Publication, 2015

Understanding the Science Behind Lipogems® Technology

Method and Device for Preparing Non-Embryonic Stem Cells

Carlo Tremolada, Carlo Ventura, Milford Graves, US Patent Application Publication, 2015

Understanding the Lipogems® Device: How Your Fat Tissue Gets Prepared for Treatment

Device and a Method for Preparing a Tissue

Carlo Tremolada, United States Patent, 2015

Understanding the Lipogems® Device: A Patented Approach to Fat Tissue Preparation

Device and Method for Preparing Tissue, Particularly Adipose Tissue

Carlo Tremolada, United States Patent, 2015

Understanding the Lipogems® Patent: A Method for Preparing Regenerative Fat Tissue

Preparation and Method for Producing a Preparation Comprising Mesenchymal Stem Cells

Carlo Tremolada, U.S. Patent Application Publication, 2015

Understanding Exosomes: How Fat Processing Methods Affect Healing Signals

Differences in exosome content of human adipose tissue processed by non-enzymatic and enzymatic methods

M. García-Contreras, F. Messaggio, O. Jimenez, A. Mendez, 2014

Lipogems Shows Promise for Restoring Blood Flow in Limb Ischemia

Lipogems, a New Modality of Fat Tissue Handling to Enhance Tissue Repair in Chronic Hind Limb Ischemia

F. Bianchi, E. Olivi, M. Baldassarre, F.A. Giannone, M. Laggetta, S. Valente, C. Cavallini, R. Tassinari, S. Canaider, G. Pasquinelli, C. Tremolada, C. Ventura, 2014

Understanding Stem Cells in Disc Disease: What This Research Reveals

Gene Expression Profile Analysis of Human Mesenchymal Stem Cells from Herniated and Degenerated Intervertebral Discs Reveals Different Expression of Osteopontin

Giovanni Marfia, Stefania Elena Navone, Clara Di Vito, Silvia Tabano, Lorenzo Gianmattei, Andrea Di Cristofori, Roberta Gualtierotti, Monica Miozzo, Paolo Rampini, Manuela Caroli, Laura Riboni, Rolando Campanella, 2014

Gentle Processing May Boost Stem Cell Healing Potential

Radioelectric Asymmetric Conveyed Fields and Human Adipose-Derived Stem Cells Obtained With a Nonenzymatic Method and Device: A Novel Approach to Multipotency

Margherita Maioli, Salvatore Rinaldi, Sara Santaniello, Alessandro Castagna, Gianfranco Pigliaru, Alessandro Delitala, Francesca Bianchi, Carlo Tremolada, Vania Fontani, Carlo Ventura, Cell Transplantation, 2014

Understanding the Science Behind Lipogems® Technology

A New Nonenzymatic Method and Device to Obtain a Fat Tissue Derivative Highly Enriched in Pericyte-Like Elements by Mild Mechanical Forces From Human Lipoaspirates

Francesca Bianchi, Margherita Maioli, Erika Leonardi, Elena Olivi, Gianandrea Pasquinelli, Sabrina Valente, Armando J. Mendez, Camillo Ricordi, Mirco Raffaini, Carlo Tremolada, Carlo Ventura, Cell Transplantation, 2013

Non-Enzymatic Fat Processing Boosts Stem Cell Potential

Radio Electric Asymmetric Conveyed Fields and Human Adipose-Derived Stem Cells Obtained with a Non-Enzymatic Method and Device

Margherita Maioli, Salvatore Rinaldi, Sara Santaniello, Alessandro Castagna, Gianfranco Pigliaru, Alessandro Delitala, Francesca Bianchi, Carlo Tremolada, Vania Fontani, Carlo Ventura, Cell Transplantation, 2013

Important Notice: This Study Does Not Relate to Lipogems®

Effects of immunosuppression on proliferation in transplanted islets

Christian Krautz, Steffen Wolk, Anja Steffen, Klaus-Peter Knoch, Uta Ceglarek, Hans-Detlev Saeger, Michele Solimena, Stephan Kersting, Cell Transplantation, 2011

Understanding the Science Behind Lipogems®: How Clinical Results Led to Laboratory Discoveries

Mesenchymal Stem Cells in Lipogems, a Reverse Story: from Clinical Practice to Basic Science

Carlo Tremolada, Camillo Ricordi, Arnold I. Caplan, Carlo Ventura

Understanding Fat Tissue as a Source of Healing Cells

Adipocyte Transplantation and Stem Cells: Plastic Surgery Meets Regenerative Medicine

Carlo Tremolada, Giancarlo Palmieri, Camillo Ricordi